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Chocolate Genetics

A warm-brown dilution of black — and it's sex-linked

Chocolate turns a bird's black into a rich, warm brown. What makes it worth its own page is not the colour but the inheritance: chocolate sits on the sex chromosome, so it behaves differently in roosters and hens — and can be used to sex chicks at hatch. For the wider system, see the Genetics Guide.

The gene: TYRP1

Chocolate (Choc) is a dilution of eumelanin — the black pigment — shifting it to a warm chocolate brown. It leaves pheomelanin (red/gold) essentially untouched, so on a gold-based bird only the black parts turn brown. Its gene has been identified as TYRP1 (tyrosinase-related protein 1), an enzyme in the black-pigment pathway; a single missense mutation changes the pigment granule's structure and gives the brown result.1

Sex-linked recessive inheritance

The important part: TYRP1 lies on the Z chromosome, so chocolate is sex-linked, and it is recessive. Because roosters are ZZ and hens are ZW:

So chocolate appears more readily in hens, and a normal-looking rooster can carry it hidden — a common source of "where did that come from?" chocolate chicks.

Chocolate can sex chicks at hatch. Because it is recessive and sex-linked, a chocolate rooster (choc/choc) over a non-chocolate hen gives chocolate daughters and normal-looking (carrier) sons — the daughters inherit their single Z, carrying chocolate, from their father. It is the recessive mirror-image of the gold/silver and barring sexing crosses on the sex-linked genes page, and you can set it up in the Breeding Outcomes tool.

Chocolate vs the other browns

Several genes can make a bird "brown", and they are not interchangeable. Chocolate is specifically the sex-linked TYRP1 dilution of black:

Brown effectGene / locusHow it differs from chocolate
Chocolate (Choc)TYRP1, Z-linked, recessive
DunAn allele of the Dominant White locus (PMEL17), autosomal, incompletely dominantDifferent gene and chromosome; not sex-linked, and one copy already shows.
Dark Brown ground (Db)Dark Brown locus, autosomalDeepens the red/gold ground colour, not the black — a different pigment entirely.

If a "brown" trait is passing differently to sons and daughters, chocolate is the likely candidate; if it shows with a single copy and isn't sex-linked, look at dun instead.

References
  1. Li J et al. (2019) A missense mutation in TYRP1 causes the chocolate plumage color in chicken and alters melanosome structure. Pigment Cell & Melanoma Research 32:381–390. Dun as a PMEL17 allele: Kerje S et al. (2004) Genetics 168:1507–1518 (see the Genetics Guide references).
Try it: set up a chocolate cross · how sex-linkage works · read the Genetics Guide